83702
Sigma
Rhodamine 123
BioChemika, for fluorescence, ≥90% (HPLC)
| Synonym: | 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester |
| CAS Number: | 62669-70-9 |
| Linear Formula: | C21H17ClN2O3 |
| Molecular Weight: | 380.82 |
| Beilstein Registry Number: | 6030951 |
| EC Number: | 263-687-8 |
| MDL number: | MFCD00012664 |
| PubChem Substance ID: | 24888136 |
Description
| Biochem/physiol Actions | Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1. |
| Other Notes | Supravital mitochondrial-specific dye1 |
Properties
| grade | for fluorescence |
| product line | BioChemika |
| assay | ≥90% (HPLC) |
| total impurities | ≤10% water |
| solubility | ethanol: 20 mg/mL, clear, red |
| suitability | in accordance for fluorescence |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
| RTECS | ZE0883000 |
References
| Cited Reference | 1. L. Benel et al. Bas. Appl. Histochem. 33, 71, (1989) |
| reference | Wuchter, C., et al. Haematologica 85, 711-721, (2000) |
| Minderman, H., et al. Cytometry 25, 14-20, (1996) |
